Effects of pH, Carbon, and Nitrogen Stress to Cytoplasmic Streaming, Hyphal Fusion, and a-amylase Secretion in Aspergillus oryza (1TDI 3002 and ITDI 3038)

Abstract

Aspergillus oryzae is a filamentous fungi that is formed by a network of hyphae used for the uptake of nutrients and secretion of enzymes. Its use in the industry for fermentation is highly dependent on the enzyme production of the organism. This study presents the effects in response to different physical and chemical stress like high pH, low pH, and depletion of carbon and nitrogen sources exhibited by A. oryzae strains ITDI 3002 and ITDI 3038, seen as changes in the cytoplasmic streaming, and the formation of anastomoses among hyphae which are possible hyphal fusions, to understand the physiological process in relation to its amylase production. Effects of the following stress a-amylase. In this study, application of the different stress resulted to the arrest of cytoplasmic streaming and fusion of hyphae in both strains, ITDI 3002 and ITDI 3038, of A. oryzae. In Ao 3038, it was observed that there was a decrease in a-amylase activity when subjected to high pH and low pH while an increase in the enzyme activity was observed when carbon and nitrogen sources were added. However, statistical analysis support that stresses applied on the strains may not have been enough to elicit significant variation between the treatments and control.