Potential Antitumor Effect of Immunostimulating Mycelia-Derived Polysaccharide from Ganodernta Lucidum (Ps-G) on Ht-29 Tumor-Induced Female Balb/C Mice

Abstract

Ganoderma lucidum has been used for centuries in cancer prevention and treatment in China and other Asian countries. This study aimed to determine if polysaccharides from G. lucidum (PS-G) extracts can elicit antitumor responses in HT-29 human colon adenocarcinoma tumor- induced female BALB/c mice. Immunosuppressed mice from control (CSS, n=6) and treatment (CPG, n=10) groups were xenografted with 1 x 106 HT-29 cancer cells. After nine days of tumor growth, the groups were treated with NSS (control) and PS-G (treatment). Tumor volume was measured once daily using a vernier caliper. The tumors were excised after five days of treatment and were cut into serial cross sections and stained with Hematoxylin & Eosin for histopathological analysis. Statistical analysis shows that there is a significant difference in the mean tumor volume before and after five days of PS-G treatment. Also, the tumor morphology of PS-G treated mice showed greater percent area of tumor necrosis (mean = 39.17%) as compared to NSS treated mice (mean = 7%). Mycelia- derived PS-G demonstrated antitumor effect and immunostimulating effect on the action rather than the proliferation of immune cells.