Screening of Some Philippine Marine Sponges for Anti-platelet Aggregation Activity

Abstract

Extracts from 17 Pliilippine sponges from the UP Marine Science collection were tested for their anti-platelet aggregation (APA) activity using the Giemsa assay. Crude methanol extracts were dried and then dissolved in dimethysulfbxide at a final concentration of 10 mg/mL. Positive APA result was characterized by 比e absence of violet gels in the wells. Only one out of the 17 specimens showed APA activity. This was a sponge coded as PK 97 3-38 by the UP-MSI Marine Natural Products Team. The thin-layer chromatography (TLC) profile of PK 97 3-38 test extract was examined to partially characterized the active components of the sponge. Further isolation and purification of the extract from the screened specimen is recommended.