Evaluation of the Anti-Platelet Aggregation Activity of the Water and Oil Extracts of Pogostemon Cablin Leaves on Rabbit Serum

Abstract

Water and oil extracts from Pogostemon cab I in leaves were collected using hydro steam distillation to evaluate their anti-platelet aggregation activity. The effect of these extracts in concentrations of 10%, 25%, 50%, 100% as inhibitors of platelet aggregation were tested on plasma collected from four rabbit blood samples. Two replicates for each concentration of extracts were subjected to a PR00012 E-max precision microplate reader assay using Giemsa dye to measure the platelet aggregation. Blood samples treated with 200 pg/mL aspirin served as positive control in this experiment while untreated blood plasma was used as the negative control. The optical densities obtained at 405 nm were utilized to estimate the percent inhibition of platelet aggregation (PIPA) of the treatments. Results showed that pure concentrations (100%) of both oil and water extracts have the highest mean PIPA values at 93.35% and 82.84% respectively, among the extract concentrations used. The pure extracts also had a higher anti-platelet aggregation activity compared to the untreated blood plasma (at p = 0.018 and p = 0.039, respectively). Varying concentrations of the oil leaf extracts were directly correlated to the anti-platelet aggregation activity of the plant (R2 = 0.98 and R2 = 0.89, respectively). The platelet aggregation inhibition characteristic of P. cablin can be used as a basis for developing plant-based compounds as an alternative for commercial anti-platelet aggregation drugs.