Quantification of the Effect of Hyperbaric Oxygen on Angiogenesis in Duck Anas luzonica (Fraser 1839) Embryos Using CAM Assay

Abstract

Wound healing researches revealed inductive effect of hyperbaric oxygen on angiogenesis. In this study, twenty Anas luzonica eggs implanted with gelfoam were divided into three groups: namely, unexposed (Control), normobaric (NB) and hyperbaric (HB). At 12 days post-laruing, NB and HB were exposed to 100% oxygen at 1-atm and 2.5-atm respectively. Egg contents were later transferred and photographed for fractal analysis while gelfoams with some underlying chorioallantoic membrane were processed for histoanalysis via planimetric point counting method. The HB group featured the highest vessel count and mean fractal index, suggesting that hyperbaric oxygen does stimulate angiogenesis to a greater degree than normobaric oxygen. Thus, hyperoxia stimulates angiogenesis by increasing metabolic rate while increased ambient pressure effects still requires further study.