Effects of Petunidin-3-Glucoside on the Lifespan and Motor Function of Drosophila Melanogaster Under the Absence and Presence of Stress Conditions

Abstract

Petunidin-3-glucoside (P3G) is an anthocyanin that can be found in vegetables, fruits, and wine. Anthocyanins are capable of reducing oxidative stress through their free-radical scavenging capacity, which can be attributed to their ring orientation. However, P3G remains to be understudied in vivo, particularly in Drosophila melanogaster models. Thus, this study tested the effects of P3G on the lifespan and motor function of D. melanogaster under the absence and presence of stress conditions, namely chronic exposure to sublethal concentrations of alcohol and glyphosate. In groups without stress, only 12.5 μg/ml concentration of P3G exhibited positive significant effects on the lifespan of D. melanogaster, while higher concentrations observed no significant difference from the control. Additionally, in the presence of chronic alcohol exposure, only 50 μg/ml concentration of P3G exhibited positive significant effects on the lifespan of D. melanogaster, while lower concentrations observed no significant difference from the control. However, P3G did not result in any significant effect on D. melanogaster under chronic glyphosate exposure. In terms of geotactic activity of D. melanogaster, P3G supplementation did not result in any significant effect under the absence and presence of stress conditions. In conclusion, P3G is likely to extend the lifespan of D. melanogaster without stress only at low concentrations and is likely to mitigate the neurotoxic effects of alcohol over glyphosate.