The Radioprotective Effects of Glo-Herbal on the Bone Marrow of Irradiated Albino Mice (Mus musculus L.)

Abstract

The radioprotective effect of Glo-Herbal was demonstrated using the micronucleus test. Four 7-week old mice (Mus musculus L.) were used for each of the six groups: negative control group, positive control group, 25%, 50%, 75% and 100% treatment groups. The negative control group . Was not given any treatment nor exposed to radiation, while the positive control group was not given any treatment but was exposed to radiation. The concentrations were administered ad libitum via drinking bottles, for 30 days. Ionizing gamma radiation (6Gy) was used to induce formation of micronucleus on the bone marrow cells of the mice. The mice sacrificed and the femori were extracted. The bone marrow cells were collected for slide preparation. One thousand polychromatic erythrocytes (PCEs) per mouse were screened. The mean frequencies of micro nucleated polychromatic erythrocytes were as follows: 36.5 +/-5.5 (positive control), 20.25 +/-4.75 (25% treatment group), 15 +/-3.5 (50% treatment group), 14.25 +/-0.875 (75% treatment group), 12 +/-2.0 (100% treatment group) and 7.5 +/-2.0 (negative control). Using the Kruskal-Wallis one way ANOVA, it was proven that the groups’ responses varied. Multiple comparisons procedure showed that there is a significant difference only between the positive and negative control group. This indicates that the effects of Glo-Herbal follow a dose-dependent pattern, but are not great enough to be considered statistically significant.