http://dspace.cas.upm.edu.ph:8080/xmlui/handle/123456789/170 Bachelor thesis of BS Biology students 2026-04-12T21:59:57Z http://dspace.cas.upm.edu.ph:8080/xmlui/handle/123456789/3591 Partial Purification and Characterization of Phenoloxidase in Coconut Beetle Larvae (Oryctes rhinoceros L.) Umali, Janice; Yao, Jocelyn Coconut beetle larvae have a protein called phenoloxidase present in their hemolymph that is essential in arthropod immune response. Prior to characterization and isolation of an enzyme designing optimum assay conditions is crucial. This will stabilize the enzyme and ensure optimal activity. In this study, phenoloxidase activity was measured as a function of varying substrates, activators with their incubation periods and incubation temperatures, and buffer pH. The substrate 4-methylcatechol appeared as the best substrate for the enzyme. Among the activators tested, trypsin efficiently activated phenoloxidase within 20-60 minutes incubation periods at 30°C in sodium phosphate buffer with pH ranging from 6.0 to 8.0. The hemolymph was partially purified for pro-phenoloxidase through DEAE and SP ion-exchange chromatography. Fractions positive for enzyme activity were loaded on SOS-PAGE. Partially purified prophenoloxidase shows a molecular weight of 85-92 kDa while its active form (phenoloxidase) has a molecular weight of 62-67 kDa. 2002-03-01T00:00:00Z http://dspace.cas.upm.edu.ph:8080/xmlui/handle/123456789/3588 A Study on the Meristic and Morphometric Characteristics of Thailand Strain and GIFT Strain of Nile Tilapia (Oreochromis niloticus) Veto, Rholine Gem Martin S.; Tismal, Daniel S. II Thirteen biometric characters were used to differentiate and compare the Thailand-strain and the GIFT-strain of Nile Tilapia (Oreochromis niloticus). Meristic and morphometric data from 30 samples for each strain were subjected to descriptive and statistical analysis. The study showed that there are no significant differences in all meristic characters (number of dorsal fin rays, number of pectoral fin rays, number of pelvic fin rays, and number or anal fin rays) as well as the horizontal eye diameter between the Thailand-strain and GIFT-strain of 0. niloticus. Generally, the GIFT-strain of 0. niloticus has greater values for the other morphometric characters (head length, preorbital distance, postorbital distance, orbital depth or the head, occipital depth of the head, total body length, standard length, and total body weight) compared to the Thailand-strain 0. niloticus 2002-02-01T00:00:00Z http://dspace.cas.upm.edu.ph:8080/xmlui/handle/123456789/3582 Effects of Cyclooxygenase Inhibitors on the Radiation Response of Intestinal Crypt Cells in Male Mice Dones, Rosa Angela SA.; Serna, Paulene-Ver A. The effects of varying doses of different types of Cyclooxygenase (COX) inhibitors on irradiated and nonirradiated murine intestinal crypt cells were investigated. Cell counts, supported by data from TUNEL assay and immunohistochemical analyses, revealed that apoptosis was greatest in irradiated tissues treated with NSAIDS. Irradiated cells treated with COX-2 inhibitors showed least occurrence of apoptosis with increased occurrence of regenerating cells. Results suggest a potential role of COX- 2 selective inhibitors as a radioprotective agent for normal cells in radiation therapy. 2002-03-01T00:00:00Z http://dspace.cas.upm.edu.ph:8080/xmlui/handle/123456789/3581 The Radioprotective Effects of Glo-Herbal on the Bone Marrow of Irradiated Albino Mice (Mus musculus L.) Dela Cruz, Aileen; Yuhico, Luke The radioprotective effect of Glo-Herbal was demonstrated using the micronucleus test. Four 7-week old mice (Mus musculus L.) were used for each of the six groups: negative control group, positive control group, 25%, 50%, 75% and 100% treatment groups. The negative control group . Was not given any treatment nor exposed to radiation, while the positive control group was not given any treatment but was exposed to radiation. The concentrations were administered ad libitum via drinking bottles, for 30 days. Ionizing gamma radiation (6Gy) was used to induce formation of micronucleus on the bone marrow cells of the mice. The mice sacrificed and the femori were extracted. The bone marrow cells were collected for slide preparation. One thousand polychromatic erythrocytes (PCEs) per mouse were screened. The mean frequencies of micro nucleated polychromatic erythrocytes were as follows: 36.5 +/-5.5 (positive control), 20.25 +/-4.75 (25% treatment group), 15 +/-3.5 (50% treatment group), 14.25 +/-0.875 (75% treatment group), 12 +/-2.0 (100% treatment group) and 7.5 +/-2.0 (negative control). Using the Kruskal-Wallis one way ANOVA, it was proven that the groups’ responses varied. Multiple comparisons procedure showed that there is a significant difference only between the positive and negative control group. This indicates that the effects of Glo-Herbal follow a dose-dependent pattern, but are not great enough to be considered statistically significant. 2002-03-01T00:00:00Z