The Use of Random Amplified Polymorphic DNAs (RAPDs) to Analyze the Genetic Variation Among Selected Oliva Species from the Visayas

Chua, Leslie Anne K.; Panlilio, Robin A.

dc.contributor.author	Chua, Leslie Anne K.
dc.contributor.author	Panlilio, Robin A.
dc.date.accessioned	2023-05-15T02:16:49Z
dc.date.available	2023-05-15T02:16:49Z
dc.date.issued	2002-04
dc.identifier.uri	http://dspace.cas.upm.edu.ph:8080/xmlui/handle/123456789/2191
dc.description.abstract	Random Amplified Polymorphic DNA (RAPD) analysis was applied to three previously recognized species of Oliva from the Visayas. Total genomic DNA was extracted from the ethanol-preserved samples following the method of Chomczynski et al. (1997) with some modifications. To assay genetic polymorphisms, the genomic DNAs were subjected to Polymerase Chain Reaction using one random primer. Different RAPD fragment patterns were observed for the different species. Analysis of the data using Popgene ver. 1.31 showed separation of the samples into four discrete groups - O. vidua, O. amythestina, O. reticulata and O. tigridella.	en_US
dc.subject	Oliva (genus of sea snails)
dc.subject	Visayas (Philippines)
dc.subject	Random Amplified Polymorphic DNA
dc.subject	Genetic polymorphism
dc.subject	Species identification
dc.title	The Use of Random Amplified Polymorphic DNAs (RAPDs) to Analyze the Genetic Variation Among Selected Oliva Species from the Visayas	en_US
dc.type	Thesis	en_US