Abstract:
The potential anti-bacterial activity of Ganoderma lucidum and
Ganoderma applanatum, macrofungi collected from Mt. Makiling, Los
Baiios, Laguna, was determined. Crude extracts of these macrofungi
were obtained using hexane as the extracting solvent. The effect of the
extracts were tested on Escherichia coli, a gram-negative bacteria and
Bacillus subtilis, a gram-positive bacteria, with streptomycin as positive
control and 95% ethanol as negative control. The anti-bacterial activity of
the crude extracts was determined using the Paper Disc Diffusion
method. G. lucidum exhibited a positive inhibitory effect, where the zone
of inhibition measured were 14.17 mm and 13.67 mm for £. coli and B.
subtilis, respectively. Statistical analysis using Analysis of Variance
showed that G. lucidum extract was not significantly different at a=0.05
from the measurements obtained using streptomycin which were 15.59
mm and 13.84 mm, for LE. coli and B. subtilis respectively. The
comparable inhibitory effect of the G. lucidum extract with streptomycin,
a commercially known anti-bacterial agent, suggests that the extract from
can be a potential antibiotic against gram-positive and gram-negative
bacteria. G. applanatum, on the other hand, did not exhibit any inhibitory
effect against the two bacteria. Similar negative results were obtained
from the set-up with ethanol as negative control.
