Abstract:
Twenty-four marine bacterial isolates from marine sponges were
screened for anti-platelet aggregation activity. Chloroform extracts of
these bacteria were tested on platelet-rich plasma and assayed for antiplatelet
aggregation using Giemsa stain. Only the extract from the culture
medium of the bacteria coded as PS51-1 isolated from the sponge
Latrunculia sp. tested positive for anti-platelet aggregation in the Giemsa
stain assay. The thin-layer chromatography (TLC) profile of the extract
from the culture medium of the P51-1 isolate showed the presence of
highly conjugated and nitrogenous compounds. The bacteria P51-1 is
gram negative, forming yellow circular elevated colonies with entire
margins in marine agar, and exhibits pellicle growth in marine broth
medium. A BIOLOG microplate assay was used to show the “metabolic
fingerprint” of PS51-1 based on its ability to utilize different carbon
sources. Results from the BIOLOG microplate and its comparison to a
bacterial database shows that P51-1’s closest carbon utilization profile
match is Vibrio harveyi.
