Screening of Some Philippine Marine Bacteria for Anti-Platelet Aggregation Activity

Abstract

Twenty-four marine bacterial isolates from marine sponges were screened for anti-platelet aggregation activity. Chloroform extracts of these bacteria were tested on platelet-rich plasma and assayed for antiplatelet aggregation using Giemsa stain. Only the extract from the culture medium of the bacteria coded as PS51-1 isolated from the sponge Latrunculia sp. tested positive for anti-platelet aggregation in the Giemsa stain assay. The thin-layer chromatography (TLC) profile of the extract from the culture medium of the P51-1 isolate showed the presence of highly conjugated and nitrogenous compounds. The bacteria P51-1 is gram negative, forming yellow circular elevated colonies with entire margins in marine agar, and exhibits pellicle growth in marine broth medium. A BIOLOG microplate assay was used to show the “metabolic fingerprint” of PS51-1 based on its ability to utilize different carbon sources. Results from the BIOLOG microplate and its comparison to a bacterial database shows that P51-1’s closest carbon utilization profile match is Vibrio harveyi.