Abstract:
The effect of wild honey and irradiated wild honey on angiogenesis in
duck Anas luzonica embryo was studied and compared using the
Chorioallantoic Membrane Assay. The degree of angiogenesis in the
chorioallantoic membranes (CAMs) of the duck embryo was determined by
fractal analysis using the box-counting method in which fractal indices of
CAMs were derived. The untreated CAMs showed the lowest degree of
angiogenesis. The CAMs inoculated with wild honey promoted similar degree
of angiogenesis with that of heparin and promoted higher degree of
angiogenesis compared to spironolactone. CAMs inoculated with irradiated
wild honey promoted a higher degree of angiogenesis compared with CAMs
treated with heparin. Promotion of angiogenesis in CAMs of duck embryos
treated with wild honey may be attributed to the production of hyaluronic acid
in CAM brought about by the glucose component of honey (Whalen and
Zetter, 1992; Knudson and Knudson, 1992). On the other hand, the highest
degree of angiogenesis in CAM of duck embryos treated with irradiated wild
honey may be attributed to the increase production of hyaluronic acid in CAM
as an effect of gamma irradiation on glucose component of the honey sample
(Whalen and Zetter, 1992; Knudson and Knudson, 1992).
