Abstract:
The effect of freeze-drying and 25 Kgy of gamma radiation on the
antibacterial action of fresh human amnions was determined by comparing
the antibacterial effect of freeze-dried and irradiated human amnions on
the Staphylococcus aureus population on rat wounds. Twenty male albino
full-thickness rat wounds were inoculated with approximately 10° S.
aureus bacterial density and treated with fresh, freeze-dried, and irradiated
human amnions 5 days post wound creation. Tissue bacterial counts were
performed on wounds treated with the various amnions and the untreated
control group on the 2™ and 4™ day post treatment. Statistical analysis
revealed a significant difference in the percent change in S. aureus
bacterial counts of the freeze-dried, and irradiated amnions compared to
that of the untreated control, indicating an antibacterial action. No
significant difference was observed in the percent change in S. aureus
bacterial counts among the fresh, freeze-dried, and irradiated human
amnions, suggesting that freeze-drying and 25 Kgy of gamma radiation
had no effect on the antibacterial action of fresh human amnions on
wounds.
