Abstract:
The radioprotective effect of Glo-Herbal was demonstrated using the
micronucleus test. Four 7-week old mice (Mus musculus L.) were used for
each of the six groups: negative control group, positive control group,
25%, 50%, 75% and 100% treatment groups. The negative control group
. Was not given any treatment nor exposed to radiation, while the positive
control group was not given any treatment but was exposed to radiation.
The concentrations were administered ad libitum via drinking bottles, for
30 days. Ionizing gamma radiation (6Gy) was used to induce formation of
micronucleus on the bone marrow cells of the mice. The mice sacrificed
and the femori were extracted. The bone marrow cells were collected for
slide preparation. One thousand polychromatic erythrocytes (PCEs) per
mouse were screened. The mean frequencies of micro nucleated
polychromatic erythrocytes were as follows: 36.5 +/-5.5 (positive control),
20.25 +/-4.75 (25% treatment group), 15 +/-3.5 (50% treatment group),
14.25 +/-0.875 (75% treatment group), 12 +/-2.0 (100% treatment group)
and 7.5 +/-2.0 (negative control). Using the Kruskal-Wallis one way
ANOVA, it was proven that the groups’ responses varied. Multiple
comparisons procedure showed that there is a significant difference only
between the positive and negative control group. This indicates that the
effects of Glo-Herbal follow a dose-dependent pattern, but are not great
enough to be considered statistically significant.
